ABSTRACT
Background: Body mass index (BMI) is being widely used to assess obesity and associated cardiovascular risk but found to be deficient ofassessing visceral obesity for which ABSI was developed. Aim and objectives were to determine a body shape index (ABSI) as a better marker than BMI in assessing visceral obesity in type 2 diabetes mellitus (T2DM) patients.Methods: The present cross-sectional study consisted total 150 patients over 40year age, both male (90) and menopaused female (60). USG was used to measure the visceral obesity.Results: The area under the ROC curve (AUROC) for BMI (kg/m²) predicting V/S fat ratio: >2.5 vs V/S fat ratio: <2.5 was 0.593 (95% CI: 0.5-0.685), thus demonstrating poor diagnostic performance compared to ABSI which was 0.815 (95% CI: 0.748-0.882), thus demonstrating good diagnostic performance. Conclusions: ABSI was better in assessing visceral obesity compared to BMI so can be used along with other markers in assessing cardiovascular risk.
ABSTRACT
Tannin acyl hydrolase commonly known as tannase is an industrially important enzyme having a wide range of applications, so there is always a scope for novel tannase with better characteristics. A newly isolated tannase-yielding fungal strain identified as Penicillium atramentosum KM was used for tannase production under solid-state fermentation (SSF) using different agro residues like amla (Phyllanthus emblica), ber (Zyzyphus mauritiana), jamun (Syzygium cumini), Jamoa (Eugenia cuspidate) and keekar (Acacia nilotica) leaves. Among these substrates, maximal extracellular tannase production i.e. 170.75 U/gds and 165.56 U/gds was obtained with jamun and keekar leaves respectively at 28ºC after 96 h. A substrate to distilled water ratio of 1:2 (w/v) was found to be the best for tannase production. Supplementation of sodium nitrate (NaNO3) as nitrogen source had enhanced tannase production both in jamun and keekar leaves. Applications of the enzyme were studied in wine clarification and tea cream solubilization. It resulted in 38.05 percent reduction of tannic acid content in case of jamun wine, 43.59 percent reduction in case of grape wine and 74 percent reduction in the tea extract after 3 h at 35ºC.
Subject(s)
Enzyme Activation , Fermentation , Hydrolases/analysis , Penicillium/enzymology , Penicillium/isolation & purification , Hydrolyzable Tannins/analysis , Hydrolyzable Tannins/isolation & purification , Catalysis , Methods , Solubility , MethodsABSTRACT
Digoxin (7.5 micrograms icv) induced 'pop-corn' type of convulsions and 100% mortality. The GABA-ergic agents produced varying degree of protection against digoxin-induced neurotoxicity. Diazepam (4 mg/kg) offered significant protection whereas pentobarbital (5 mg/kg) and baclofen (5 mg/kg) markedly reduced per cent mortality, but ethanol (2 g/kg), progabide (50 mg/kg) and muscimol (0.5 mg/kg) as well as GABA (50 mg/kg) could not offer significant protection in doses used. GABA-ergic agonists; GABA, baclofen, diazepam and pentobarbital when administered along with MK-801 (0.5 mg/kg) a non-competitive NMDA antagonist, a potentiation of anticonvulsant action of MK-801 was observed. MK-801 showed potent anticonvulsant profile in dose range (0.25-1 mg/kg) studied. A synergistic influence of Mg2+ and K+ ions on NMDA receptor antagonism was also observed. A role of GABA-ergic facilitation and NMDA antagonism as a potential anticonvulsant approach in digoxin-induced convulsions in rats has been suggested.